![]() This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Ĭompeting interests: The authors have declared that no conflicts of interest exist.Īdenovirus- β-galactosidase CDSC-Pax7 ctx,ĭevelopmental Studies Hybridoma Bank HAN-puro, Received: JanuAccepted: FebruPublished: May 11, 2004Ĭopyright: © 2004 Seale et al. PLoS Biol 2(5):Īcademic Editor: Jon Epstein, University of Pennsylvania Moreover, these experiments suggest that viral transduction of Pax7 is a potential therapeutic approach for the treatment of neuromuscular degenerative diseases.Ĭitation: Seale P, Ishibashi J, Scimè A, Rudnicki MA (2004) Pax7 Is Necessary and Sufficient for the Myogenic Specification of CD45 +:Sca1 + Stem Cells from Injured Muscle. ![]() Taken together, these results indicate that Pax7 is necessary and sufficient to induce the myogenic specification of CD45 + stem cells resident in adult skeletal muscle. Notably, infection of Pax7-deficient muscle with adenoviral Pax7 resulted in the de novo formation of regenerated myofibers. Infection of CD45 −:Sca1 − cells from Pax7 −/− muscle similarly gave rise to myoblasts. The resulting myoblasts expressed Myf5 and MyoD and differentiated into myotubes that expressed myogenin and myosin heavy chain. ![]() Infection of CD45 +:Sca1 + cells from uninjured muscle with retrovirus expressing Pax7 efficiently activated the myogenic program. ![]() By contrast, CD45 +:Sca1 + isolated from injured Pax7 −/− muscle were incapable of forming myoblasts. CD45 +:Sca1 + adult stem cells isolated from uninjured muscle do not display any myogenic potential, whereas those isolated from regenerating muscle give rise to myoblasts expressing the paired-box transcription factor Pax7 and the bHLH factors Myf5 and MyoD. ![]()
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